CAS No.: 54827-17-7
Product name: 3,3',5,5'-Tetramethylbenzidine
Abbreviation: TMB
Appearance: White to pale white powder
Purity(HPLC): ≥99%
Molecular formula: C16H20N2
Molecular weight: 240.34
Package: In Aluminum foil bag, cartons outside.
Storage: Preserve in a well-closed container and keep in cool, dry place, avoid light.
Biochemical analysis: 3,3',5,5'-Tetramethylbenzidine is commonly used as a substrate for enzymatic reactions in biochemical assays. It can produce a detectable color reaction through enzymatic action and is used to determine enzyme activity and concentration.TMB is a new and safe chromogenic reagent. Compared with traditional chromogenic reagents such as benzidine, o-biphenylamine and o-phenylenediamine, TMB has the advantages of high detection sensitivity and good stability, and is safe to use. The results of animal test and Ames bacterial auto-mutagenicity test show that TMB is non-carcinogenic and non-mutagenic.
Chemical analysis: 3,3',5,5'-Tetramethylbenzidine can be used as an indicator for iron ions in spectrophotometric methods, it will complex with iron to produce a chromogenic product, which can be used for quantitative analysis of iron ions.
Chemical reagent: 3,3',5,5'-Tetramethylbenzidine can also be used as the raw material of dyes and indicators, and has certain applications in organic synthesis and chemical experiments.