Heynova (Shanghai) New Material Technology CO., Ltd.
Heynova (Shanghai) New Material Technology CO., Ltd.
3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7 C16H20N2

3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7 C16H20N2

CAS No.: 54827-17-7

Product name: 3,3',5,5'-Tetramethylbenzidine

Abbreviation: TMB

Appearance: White to pale white powder

Purity(HPLC): ≥99%

Molecular formula: C16H20N2

Molecular weight: 240.34

Package: In Aluminum foil bag, cartons outside.

Storage: Preserve in a well-closed container and keep in cool, dry place, avoid light.

3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7 C16H20N2

Application of 3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7 C16H20N2


  • Biochemical analysis: 3,3',5,5'-Tetramethylbenzidine is commonly used as a substrate for enzymatic reactions in biochemical assays. It can produce a detectable color reaction through enzymatic action and is used to determine enzyme activity and concentration.TMB is a new and safe chromogenic reagent. Compared with traditional chromogenic reagents such as benzidine, o-biphenylamine and o-phenylenediamine, TMB has the advantages of high detection sensitivity and good stability, and is safe to use. The results of animal test and Ames bacterial auto-mutagenicity test show that TMB is non-carcinogenic and non-mutagenic.


  • Chemical analysis: 3,3',5,5'-Tetramethylbenzidine can be used as an indicator for iron ions in spectrophotometric methods, it will complex with iron to produce a chromogenic product, which can be used for quantitative analysis of iron ions.


  • Chemical reagent: 3,3',5,5'-Tetramethylbenzidine can also be used as the raw material of dyes and indicators, and has certain applications in organic synthesis and chemical experiments.

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3,3',5,5'-Tetramethylbenzidine (TMB) 54827-17-7 C16H20N2
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